![]() ![]() Before using the microscope or whenever an objective is changed, check that the phase rings are aligned.ĭ. Microscopes should be kept covered and the lights turned down when not in use. Inverted phase contrast microscopes are used for visualizing the cells. The humidity must also be maintained for those cells growing in tissue culture dishes so a pan of water is kept filled at all times.Ĭ. Cells should be left out of the incubator for as little time as possible and the incubator doors should not be opened for very long. Culture flasks should have loosened caps to allow for sufficient gas exchange. The cells are grown in an atmosphere of 5-10% CO2 because the medium used is buffered with sodium bicarbonate/carbonic acid and the pH must be strictly maintained. Keep the hood as free of clutter as possible because this will interfere with the laminar flow air pattern.ī. Wipe down all surfaces with ethanol before and after each use. The hoods should be turned on about 10-20 minutes before being used. Do not put your hands or face near the hood when the UV light is on as the short wave light can cause skin and eye damage. The hoods are equipped with a short-wave UV light that can be turned on for a few minutes to sterilize the surfaces of the hood, but be aware that only exposed surfaces will be accessible to the UV light. They should also never be used for bacterial or fungal work. NOTE: these are not fume hoods and should not be used for volatile or explosive chemicals. In a vertical hood, the filtered air blows down from the top of the cabinet in a horizontal hood, the filtered air blows out at the operator in a horizontal fashion. Both types of hoods have continuous displacement of air that passes through a HEPA (high efficiency particle) filter that removes particulates from the air. Horizontal hoods are designed such that the air flows directly at the operator hence they are not useful for working with hazardous organisms but are the best protection for your cultures. The vertical hood, also known as a biology safety cabinet, is best for working with hazardous organisms since the aerosols that are generated in the hood are filtered out before they are released into the surrounding environment. There are two types of laminar flow hoods, vertical and horizontal. Primary cell cultures typically will have a finite life span in culture whereas continuous cell lines are, by definition, abnormal and are often transformed cell lines. ![]() Cell cultures are derived from either primary tissue explants or cell suspensions. Tissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often used interchangeably. I Types of cells grown in culture II Work area and equipment III Preservation and storage IV Safety considerations I. Use the following link to learn more about working with cells The tutorial provides a basic overview about the study of cells that includes some background information reviews cell theory, cell sizes, and some basic information about different types of microscopes used to study cells. The following web link will take you to the Arizona Biology Project’s Studying Cells Tutorial. INTRODUCTION: Studying Cells – The Arizona Biology Project ![]() This topic part has one section: Content Tutorial. This is Part A, Intro to Cell Culture, under the module topic, Cell Culture Techniques. ![]()
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